Page 12 - Journal of Laparoscopic Surgery

Anburaja Mahalingam et al
were applied to the spermatic artery-vein plexus by using values on day 7. A significant increase (p < 0.01) in catalase
clip applicator. Two ports at paramedian site, either side of was observed immediately after operation and on 1st and
inguinal region, 4 to 6 cm lateral to mid-ventral line was 3rd postoperative days in both the groups. Thereafter, the
found easy for laparoscopic sterilization. In all the animals of values of catalase enzymes decreased and returned to base
both groups monopolar coagulation current of 60 W was used line values on day 7 postoperatively. Values of SOD enzyme
and found effective for electrocautery as well as coagulation decreased nonsignificantly (p > 0.05) upto 3rd postoperative
of the line of cutting of vas deferens. The visualization of day in both the groups. The values increased on subsequent
vas deferens was better with trendelenburg position of the intervals and reached to base values on day 7 postopera-
animals. All the animals were closely monitored for effective tively in both the groups. A significant increase (p < 0.05)
hemostasis before the final withdrawal of telescope. in reduced glutathione values upto day 1 was observed in
both the groups. Later on, the values decreased and returned
Clinical observations to base line values on day 7 postoperatively. No significant

All the animals were recovered smoothly from anesthesia. change (p > 0.05) was observed in ceruloplasmin level at
Return to normal appetite within 8 to 10 hours after recovery any postoperative days. However, transient mild elevation
from anesthesia was observed in all animals. Urination and (p > 0.05) of this protein was observed up to 7th postopera-
defecation were normal throughout the observation period. tive day in both the groups.
No significant change (p > 0.05) in respiration rate, heart rate hormonal estimation
and rectal temperature was observed. The values remained
within the normal limits, without any significant change The mean ± SE values of cortisol and testosterone are presen-
(p > 0.05) from base values at any postoperative time inter- ted in Table 4. A nonsignificant increase (p > 0.05) in
vals (Table 1). cortisol values was observed immediately after operation in
group I. However, significant increase (p < 0.01) in cortisol
hematobiochemical observations level was observed immediately after operation in group II.
On subsequent intervals, the cortisol level reduced and
Differential leukocyte count of both the groups revealed a returned to base values on day 7 in both the groups. No
significant increase (p < 0.05) in neutrophils on 3rd post- significant change (p > 0.05) in testosterone was observed
operative day with comparative lymphopenia. No signi- at different postoperative time intervals in group I. In
ficant change (p > 0.05) was observed when comparisons group II, significant decrease (p < 0.01) in testosterone level
were made between the groups at different time intervals. was observed immediately after operation and there after
Significant increase (p < 0.05) in alkaline phosphatase (ALP) the values showed gradual decrease (p > 0.05) up to 7th
level was observed on 3rd postoperative day in animals of postoperative day.
both the groups. Later on, it reduced and returned to base
values on day 7 (Table 2). The acid phosphatase (ACP) DISCUSSIoN
level showed a significant increase (p < 0.05) on 1st and The laparoscopic surgical techniques in both human and
3rd postoperative days (see Table 2).
veterinary medicine have grown tremendously. Laparoscopy
has been used to the routine diagnostic and therapeutic
estimation of oxidative Stress
methods used especially in small animals. Therefore at
The mean ± SE values of lipid peroxidation, catalase, super- present, laparoscopes provide a true panoramatic picture of
oxide dismutase reduced glutathione, ceruloplasmin are presen- the observed cavity. In addition to that, they can magnify
ted in Table 3. There was a mild, transient nonsignificant to a certain degree, what makes visualization more precise
increase (p > 0.05) in LPO values after operation up to 3rd and the picture brighter. For this reason, at using the laparo-
postoperative day in both the groups and it returned to base scopic techniques impairment of tissues hardly occurs. Since,

Table 1: Mean ± SE value of respiration rate, heart rate and rectal temperature recorded at different time intervals
Parameters Groups Before Immediately Day 1 Day 3 Day 5 Day 7
operation after operation PO PO PO PO
Respiration rate I 24.20 ± 1.28 18.80 ± 0.58 25.40 ± 0.51 24.40 ± 0.75 24.40 ± 0.93 22.60 ± 0.81
(breaths/min) II 26.00 ± 0.89 20.60 ± 0.68 24.40 ± 0.93 24.80 ± 1.66 24.60 ± 1.57 22.60 ± 1.03
Heart rate I 117.80 ± 5.00 110.60 ± 5.04 116.60 ± 3.23 116.60 ± 4.01 118.20 ± 4.08 115.20 ± 4.27
(beats/min) II 115.40 ± 8.78 110.40 ± 7.39 108.40 ± 7.71 109.40 ± 6.37 112.00 ± 6.32 115.60 ± 5.78
Rectal tempe- I 101.30 ± 0.25 101.10 ± 0.24 101.10 ± 0.40 101.10 ± 0.19 100.50 ± 0.16 100.60 ± 0.29
rature (ºF) II 101.30 ± 0.41 101.20 ± 0.25 101.50 ± 0.35 101.10 ± 0.29 100.90 ± 0.29 101.00 ± 0.16

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