Page 27 - World Journal of Laparoscopic Surgery

Bacteriological Profile of Bile
Table 2: Antibiogram of the bacteria isolated
Resistance
Klebsiella Pseudomonas Salmonella Acinetobacter
Antibiotic E. coli spp. spp. spp. spp. S. aureus
Piperacillin 43 (100%) 17 (100%) 4 (100%) 2 (100%) 1 (100%) –
Ceftazidime 22 (51.2%) 17 (100%) 4 (100%) 1 (50%) 1 (100%) –
Ceftriaxone 22 (51.2%) 17 (100%) 4 (100%) 1 (50%) 1 (100%) 1 (100%)
Cefoperazone 22 (51.2%) 17 (100%) 4 (100%) 1 (50%) 1 (100%) 1 (100%)
Gentamicin 43 (100%) 10 (58.8%) 3 (75%) 2 (100%) 1 (100%) –
Amikacin 43 (100%) 10 (58.8%) 3 (75%) 2 (100%) 1 (100%) –
Ciprofloxacin 43 (100%) 14 (82.3%) 2 (50%) 2 (100%) 1 (100%) 1 (100%)
Levofloxacin 43 (100%) 14 (82.3%) 2 (50%) 2 (100%) 1 (100%) 1 (100%)
Imipenem 0 8 (47%) 1 (25%) 0 1 (100%) 0
Meropenem 0 8 (47%) 1 (25%) 0 1 (100%) 0
Chloramphenicol 0 – – 0 – –
Cefixime – – – – – 1 (100%)
Colistin 0 0 0 0 0 –
Polymyxin B 0 0 0 0 0 –
Piperacillin/Tazobactam 0 10 (58.8%) 1 (25%) 0 0 –
Ceftriaxone/Sulbactam 0 10 (58.8%) 1 (25%) 0 0 –
Cefepime/Tazobactam 0 10 (58.8%) 1 (25%) 0 0 –
Doxycycline – – 4 (100%) – 0 –
Aztreonam – – 0 – – –
Erythromycin – – – – – 1 (100%)
Clindamycin – – – – – 1 (100%)
Linezolid – – – – – 0
Teicoplanin – – – – – 0
Vancomycin – – – – – 0
Rifampicin – – – – – 0

dIscussIon Our study was similar to Gupta et al., Khalid Anjum et al.,
This study includes 126 patients admitted to our surgery unit over Kumar et al., Manan et al., Bhansali et al., Flores et al., Pratik et al.,
2,11,13,16,19–23
and Fuks et al.
a period of 1 year. In this, 54% bile samples showed positive bile
culture. The data showed high prevalence in western Uttar Pradesh.
Our finding contrasts with other researchers. They reported very conclusIon
low prevalence. 10–12 Normally, bile is sterile in the gallbladder in the absence of gallstone
In this study, most of the cases were of middle-age-group that or any pathology of the biliary tract. There is high incidence of
is between 41 and 60 years, that is similar to other studies. Chuttani bacteribilia in cases of the inflamed gallbladder with gallstones
et al. reported maximum incidence of cholelithiasis in-between or biliary tract obstruction. Gram-negative organisms are more
31 and 60 years. 3–6,13 In our study, 17% were males and 83% were common in bile infection as they are part of normal GI flora and
females, and male-to-female ratio was 1:2.71. Similar female pre- may cause ascending infection in the gallbladder. Drug resistance
dominance has been reported by many researchers. 11–16 The most is a growing health problem, nowadays, undue and inappropriate
common organism isolated in bile culture was E. coli 63.2%, and use of antibiotics are the main cause of growing drug resistance.
the second most common was Klebsiella spp. in 25% of the patients.
Our findings were similar to other studies published by Capoor et al., references
Bhansali et al., Cristina et al., Sharma et al., Pratik et al., and many
more, the most common organism isolated was E. coli followed by 1. Gill HS, Sandhu GS, Luna A, et al. Bacteriological profile of bile in
patients undergoing cholecystectomy. Sch Acad J Biosci 2016;4(6):
Klebsiella spp. As E. coli and Klebsiella both are the most common 520–525. DOI: 10.21276/sajb.2016.4.6.12.
bacteria isolated in the bile culture as they are the commonest 2. Gupta J, Gupta A, Gupta R, et al. Bacteriological profile of bile
bacteria found in GIT and infection to the biliary system comes and antibiogram in cholecystectomy patients. J Med Sci Clin Res
from the GIT. 17–22 2017;5(4):20786–20789. DOI: 10.18535/jmscr/v5i4.169.

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