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Bacteriological Profile of Bile























            Figs 1A to C: (A) Gallbladder with multiple stones; (B) Intact gallbladder; and (C) Cholesterosis of gallbladder
























            Fig. 2:  Sex distribution                          Fig. 3:  Distribution of surgical treatment procedure

            Table 1:  Age-group-wise distribution
             Sl. no.           Age-group              Total %
             1.                  01–20               01 (0.8%)
             2.                  21–40               22 (17.4%)
             3.                  41–60               78 (62%)
             4.                  >61                 25 (19.8%)


            was 34 (17%). We have included all ages of patients in our study,
            in which maximum patients were in 41–60 age-group, 78 (62%)
            followed by 25 (19.8%) in the age-group >61, 22 (17.4%) in 21–40
            age-group, and 1 (0.8%) in 01–20 age-group (Figs 1 and 2, Table 1).
            Out of 126 patients, 96 (76.2%) patients underwent laparoscopy
            cholecystectomy, in 18 (14.3%) patients, open cholecystectomy
            was performed, and in 12 (9.5%), laparoscopy surgery was converted
            to open surgery (Fig. 3).                          Fig. 4:  Bacteriological profile of bile
               Out of 126 bile samples for culture and sensitivity, only 68
            (54%) samples were culture-positive. E. coli 43 (63.2%) was the   All Gram-negative bacterial isolates showed maximum sen-
            most common isolate followed by Klebsiella spp. 17 (25%). Other   sitivity toward imipenem, meropenem, piperacillin/tazobactam,
            organisms isolated were 4 (6%) Pseudomonas spp., 2 (3%) Salmonella   cefepime, and ceftriaxone/sulbactam. No organism was found to be
            spp. and Staphylococcus aureus, and Acinetobacter 1 (1.4%) each.     resistance to colistin and polymyxin B. Among gram-positive, bacte-
            The remaining bile samples 58 (46%) were sterile (Fig. 4).  rial isolates showed sensitivity to linezolid and teicoplanin (Table 2).


            212   World Journal of Laparoscopic Surgery, Volume 15 Issue 3 (September–December 2022)
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